This case study is intended for programme managers and health professionals interested in learning about male involvement in the context of PMTCT programmes. It reviews a multi-faceted intervention launched by the Optimizing HIV Treatment Access for Pregnant and Breastfeeding Women (OHTA) to increase male involvement in three regions in Uganda (East Central, North East, and South West). By implementing both community and facility-based activities focused on strengthening male involvement as part of a comprehensive PMTCT strategy, the programme aims to strengthen support for male participation in antenatal care (ANC), HIV testing, and HIV treatment.