This technical brief updates previous guidance from 2019 and aligns with the latest recommendations from UN agencies and partners. Global Fund support focuses on 15- to 24-year-olds in areas with moderate to high HIV incidence. While the brief emphasizes HIV prevention, it recognizes adolescents' comprehensive health needs and vulnerabilities, including harmful gender norms, inequalities, violence, and exploitation. Addressing these challenges involves strengthening primary health care, community systems, and human rights, as outlined in the Global Fund's 2023-2028 Strategy. The brief underscores the importance of tailoring interventions to the diverse needs and vulnerabilities of AGYW sub-populations, considering factors such as age and stage of life.